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MCELNs and CELNs elicited apoptosis in TNBC cells. (A)–(B) Flow cytometry detection of apoptosis in MDA-MB-231 cells treated with MCELNs and CELNs for 24 h. (C)–(D) Antagonism of MPA on cell apoptosis induced by MCELNs. (E)–(L) Western blot measured the expression levels of full length <t>PARP1,</t> cleaved PARP1, and cleaved caspase 3 following the treatment of MDA-MB-231 cells with MCELNs for 24 h ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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MCELNs and CELNs elicited apoptosis in TNBC cells. (A)–(B) Flow cytometry detection of apoptosis in MDA-MB-231 cells treated with MCELNs and CELNs for 24 h. (C)–(D) Antagonism of MPA on cell apoptosis induced by MCELNs. (E)–(L) Western blot measured the expression levels of full length PARP1, cleaved PARP1, and cleaved caspase 3 following the treatment of MDA-MB-231 cells with MCELNs for 24 h ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Journal: Materials Today Bio

Article Title: Macrophage membrane coating enhances the therapeutic effects of Houttuynia cordata exosome-like nanovesicles against triple-negative breast cancer

doi: 10.1016/j.mtbio.2025.102604

Figure Lengend Snippet: MCELNs and CELNs elicited apoptosis in TNBC cells. (A)–(B) Flow cytometry detection of apoptosis in MDA-MB-231 cells treated with MCELNs and CELNs for 24 h. (C)–(D) Antagonism of MPA on cell apoptosis induced by MCELNs. (E)–(L) Western blot measured the expression levels of full length PARP1, cleaved PARP1, and cleaved caspase 3 following the treatment of MDA-MB-231 cells with MCELNs for 24 h ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: The main materials used in this study included P-CHK1, P-ATR, CDK4, CDC25C, P21, γ-H2AX (Abclone), cleaved PARP1, full length PARP1, cleaved caspase 3, BSA (BioFroxx), BCA Protein Concentration Assay Kit (Biyotime Shanghai), SDS-PAGE protein loading buffer (BOSTER, Wuhan), Three-color prestained protein standards (Abclone), Cell Cycle Assay Kit (RedFluorescence) (Biyotime Shanghai), enhanced RIPA lysate (BOSTER, Wuhan), RPMI 1640 medium(Gibco, USA), PKH67 (MedChemExpress, USA), PEG8000 (Chron Chemicals), 5-Fluorouracil (MedChemExpress, USA), and Camptothecin (MedChemExpress, USA).

Techniques: Flow Cytometry, Western Blot, Expressing